Some operators that preserve the locality of a pseudovariety of semigroups

It is shown that if V is a local monoidal pseudovariety of semigroups, then K(m)V, D(m)V and LI(m)V are local. Other operators of the form Z(m)(_) are considered. In the process, results about the interplay between operators Z(m)(_) and (_)*D_k are obtained.Comment: To appear in International Journal of Algebra and Computatio

It comes from the sea: macroalgae-derived bioactive compounds with anti-cancer potential

Nature derived compounds represent a valuable source of bioactive molecules with enormous potential. The sea is one of the richest environments, full of skilled organisms, where algae stand out due to their unique characteristics. Marine macroalgae adapt their phenotypic characteristics, such as chemical composition, depending on the environmental conditions where they live. The compounds produced by these organisms show tremendous potential to be used in the biomedical field, due to their antioxidant, anti-inflammatory, immunomodulatory, and anti-cancer properties. Cancer is one of the deadliest diseases in the world, and the lack of effective treatments highlights the urgent need for the development of new therapeutic strategies. This review provides an overview of the current advances regarding the anti-cancer activity of the three major groups of marine macroalgae, i.e., red algae (Rhodophyta), brown algae (Phaeophyceae), and green algae (Chlorophyta) on pancreatic, lung, breast, cervical, colorectal, liver, and gastric cancers as well as leukemia and melanoma. In addition, future perspectives, and limitations regarding this field of work are also discussed.info:eu-repo/semantics/publishedVersio

Oligonucleotide sequences forming short self-complimentary hairpins can expedite the down-regulation of Coprinopsis cinerea genes

Gene silencing in fungi is often induced by dsRNA hairpin forming constructs the preparation of which can require multiple cloning steps. To simplify gene silencing in the filamentous fungi we have evaluated a high throughput cloning method for target sequences using the homobasidiomycete Coprinopsis cinerea, the GFP reporter and a commercially available vector system. The pSUPER RNAi System™, which was developed for mammalian experiments, exploits the human H1 Polymerase III (Pol III) RNA gene promoter and expedites cloning/expression of specific user-defined oligonucleotide sequences to form short self-complimentary hairpins. Transformation of C. cinerea with pSUPER constructs harboring specific oligonucleotides (19 nt stem length) enabled recovery of transformants with reduced transcripts of the GFP transgene, that were less fluorescent in protein assays and microscopic phenotypes. This technological advance should expedite functional genomic studies in C. cinerea and has wider potential for utility in other homobasidiomycete and filamentous fungi

Intravenous infusion of magnesium sulfate and its effect on horses with trigeminal-mediated headshaking.

BackgroundTrigeminal-mediated headshaking results from low-threshold firing of the trigeminal nerve resulting in apparent facial pain. Magnesium may have neuroprotective effects on nerve firing that potentially dampen signs of neuropathic pain. This hypothesis has not been investigated in horses with trigeminal-mediated headshaking.ObjectiveTo investigate head-shaking behavior in affected horses after IV magnesium sulfate infusion.AnimalsSix geldings with trigeminal-mediated headshaking.MethodsProspective randomized crossover study. Horses were controlled for diet and infused IV with 5% dextrose solution (DS; control solution at 2 mL/kg body weight [BW]) and MgSO4 50% solution (MSS at 40 mg/kg BW). Head-shaking behavior was recorded at times T0 (baseline, before infusion) and T15, T30, T60, and T120 minutes post-infusion. Venous blood variables such as pH, HCO3 - , standard base excess (SBE), Na+ , Cl- , K+ , Ca2+ , Mg2+ , total magnesium (tMg), glucose, and lactate were measured; strong ion difference (SID) and anion gap (AG) were calculated for each time point.ResultsBlood variables including pH, Na+ , Cl- , K+ , SID, AG, lactate, Ca2+ , tMg, and Mg2+ had significant changes with MSS as compared to DS treatment. Glucose, SBE, and HCO3 - did not have significant changes. A 29% reduction in head-shaking rate occurred after MSS treatment but no change occurred after DS treatment.Conclusions and clinical importanceAdministration of MSS IV increased plasma total and ionized magnesium concentrations and significantly decreased head-shaking behavior in horses with trigeminal-mediated headshaking

Glucose-evoked Na+,K+-ATPase modulation in pancreatic ß-cells from normal and impaired glucose tolerance: role of AMPK

Na+,K+-ATPase is regulated by glucose in pancreatic ß-cells, a process that is altered in glucose impaired tolerance. Although AMP dependent protein kinase (AMPK), a metabolic sensor, is believed to be central in the signal transduction cascade underlying the Na+,K+-ATPase regulation in pancreatic β-cells, its role remains unknown. The aim of this work was to clarify the role of AMPK in glucoseevoked inhibition of Na+,K+-ATPase and to evaluate whether AMPK is differently regulated in pancreatic β-cells from subjects with normal and impaired glucose tolerance. Pancreatic β-cells or islets from normal (control) or glucose-intolerant Wistar rats (GIR) were isolated and cultured. After a pre-incubation (30min) with 2.1mM glucose (G2), batches were challenged for 20min with 2.1 or 8.4mM glucose (G8) in the presence or absence of AMPK agonist (AICAR, 1mM) and antagonist (Compound C (CC), 10μM). Na+,K+-ATPase activity was assessed by quantification of Pi, in the absence and in presence of 1mM ouabain. Phosphorylation levels of α1 subunit of Na+,K+-ATPase- (Ser-23) and αAMPK-(Thr-172) was evaluated by Western blot (WB). In G2 Na+,K+-ATPase activity from normal and GIR β-cell was similar (0.184±0.030 and 0.186±0.020 μmolPi/min/mgProt, respectively). Challenging the β-cells with G8 evoked a lower inhibition of Na+,K+- ATPase activity in GIR (40%) compared to controls (62%). In control β-cell, AICAR abolished glucoseinduced Na+,K+-ATPase inhibition (0,166±0.011 μmolPi/min/mg) whereas CC had no effect. In the contrast, CC significantly potentiated glucose-evoked inhibition of Na+,K+-ATPase in GIR β-cells, reaching values similar to the controls (66%), For both GIR and control islets, G8 induced a 50% decrease of AMPK phosphorylation level compared to G2. CC mimicked the effect of G8, but was less efficient in GIR. Concomitantly, α1-Na+,K+-ATPase-(Ser-23) phosphorylation level was increased upon G8 or CC stimulation, compared to G2 or AICAR. These results suggest that AMPK plays a key role in the signaling mechanism underlying glucoseinduced modulation of the pump, a process dependent on phosphorylation cascades, and that the defect in GIR must be upstream of AMPK. Glucose-induced inhibition of Na+,K+-ATPase may result from AMPK inhibition by the fuel metabolism and subsequent activation of PKC, known to phosphorylate α1-Na+,K+-ATPase-(Ser-23). This mechanism is impaired in GIR, thus potentially contributing to the impaired glucose-induced insulin secretion in IGT. Occurring prior to overt type 2 diabetes, this might be a feature in the disease development

Offshore wind field: Application of statistical models as a spatial validation technique

Generally, atmospheric mesoscale models are used as tools to perform wind atlases. In recent decades, significant efforts have been applied to the development and improvement of this kind of models to reduce their systematic errors. These ones are assessed when model results are compared with observations. In practice, such errors could be statistically corrected if observational data was available for the same area. A deviation matrix of the wind field between WRF (Weather Research and Forecasting) mesoscale model and wind data retrieved from the QuiKSCAT satellite was obtained by the application of two statistical techniques – kriging interpolation and composite method. The spatial validation performance was evaluated with observational wind data from an anemometric mast installed on Berlengas islet since November 2006 to the present. The following are a preliminary assessment of the statistical methods as spatial validation techniques. These are a part of the spatial validation methodology to be used within the EU FP7 NORSEWInD project

Pattern based GUI testing for mobile applications

This paper presents a study aiming to assess the feasibility of using the Pattern Based GUI Testing approach, PBGT, to test mobile applications. PBGT is a new model based testing approach that aims to increase systematization, reusability and diminish the effort in modelling and testing. It is based on the concept of User Interface Test Patterns (UITP) that contain generic test strategies for testing common recurrent behaviour, the so-called UI Patterns, on GUIs through its possible different implementations after a configuration step. Although PBGT was developed having web applications in mind, it is possible to develop drivers for other platforms in order to test a wide set of applications. However, web and mobile applications are different and only the development of a new driver to execute test cases over mobile applications may not be enough. This paper describes a study aiming to identify the adaptations and updates the PBGT should undergo in order to test mobile applications. © 2014 IEEE

Educação para a liberdade

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Cupressaceae Pollen in the City of Évora, South of Portugal: Disruption of the Pollen during Air Transport Facilitates Allergen Exposure

Research Highlights: Daily airborne Cupressaceae pollen disruption ranged from 20 to 90%; relative humidity (RH), rainfall and atmospheric pressure (AtP) were the major meteorological determinants of this phenomenon. Background and Objectives: Cupressaceae family includes several species that are widely used as ornamental plants pollinating in late winter-early spring and might be responsible for allergic outbreaks. Cupressaceae pollen disruption may favour allergen dissemination, potentiating its allergenicity. The aim of this work was to characterize the Cupressaceae pollen aerobiology in Évora, South of Portugal, in 2017 and 2018, particularly the pollen disruption, and to identify the meteorological parameters contributing to this phenomenon. Materials and Methods: Pollen was collected using a Hirst type 7-day pollen trap and was identified following the standard methodology. Temperature, RH, rainfall, global solar radiation (Global Srad), AtP, wind speed and direction were obtained from a weather station installed side-by-side to the Hirst platform. Back trajectories (12-h) of air masses arriving at Évora were calculated using the HYSPLIT model. Results: Cupressaceae pollen index was higher in 2017 compared to 2018 (>5994 and 3175 pollen/m3, respectively) and 36 ± 19% (2017) and 64 ± 17% (2018) of the pollen was disrupted. Higher levels of disrupted pollen coincided with RH > 60% and rainfall. Temperature, Global Srad and AtP correlated negatively with pollen disruption. Wind speed and wind direction did not significantly correlate with pollen disruption. Intra-diurnal pollen pattern peaked between 9:00 am–2:00 pm, suggesting local origin, confirmed by the back trajectory analysis. Intra-diurnal pollen disruption profile followed hourly pollen pattern and it negatively correlated with AtP, temperature and Global Srad but was uncorrelated with RH. Conclusions: The results suggest that RH, rainfall and AtP are the main factors affecting airborne Cupressaceae pollen integrity and in conjunction with daily pollen concentration may be used to predict the risk of allergy outbreaks to this pollen type

A Transcriptomic Approach to the Recruitment of Venom Proteins in a Marine Annelid

CEECIND/02699/2017The growing number of known venomous marine invertebrates indicates that chemical warfare plays an important role in adapting to diversified ecological niches, even though it remains unclear how toxins fit into the evolutionary history of these animals. Our case study, the Polychaeta Eulalia sp., is an intertidal predator that secretes toxins. Whole-transcriptome sequencing revealed proteinaceous toxins secreted by cells in the proboscis and delivered by mucus. Toxins and accompanying enzymes promote permeabilization, coagulation impairment and the blocking of the neuromuscular activity of prey upon which the worm feeds by sucking pieces of live flesh. The main neurotoxins ("phyllotoxins") were found to be cysteine-rich proteins, a class of substances ubiquitous among venomous animals. Some toxins were phylogenetically related to Polychaeta, Mollusca or more ancient groups, such as Cnidaria. Some toxins may have evolved from non-toxin homologs that were recruited without the reduction in molecular mass and increased specificity of other invertebrate toxins. By analyzing the phylogeny of toxin mixtures, we show that Polychaeta is uniquely positioned in the evolution of animal venoms. Indeed, the phylogenetic models of mixed or individual toxins do not follow the expected eumetazoan tree-of-life and highlight that the recruitment of gene products for a role in venom systems is complex.publishersversionpublishe